WEBINAR | Tailored Cryo-EM and Correlative Workflows for Challenging Biological Projects

Advanced electron microscopy has become an essential tool for addressing complex biological questions - from fragile cell-cell interactions to intracellular structural organization. Achieving meaningful results requires more access to instrumentation. It demands integrated workflows, methodological innovation, and close collaboration between modern core facility experts and research teams. 

In this webinar, Vlada Filimonenko, Marketa Dalecka, and Dominik Pinkas from the Electron Microscopy Core Facility at the Institute of Molecular Genetics of the Czech Academy of Sciences in Prague will present how their open-access facility supports technically demanding scientific projects within the Czech BioImaging and Euro-BioImaging infrastructures. 

Through two advanced case studies, they will demonstrate how cryo-fluorescence microscopy, cryo-FIB-SEM, FIB-SEM tomography, cryo electron diffraction, and tailored sample preparation strategies enable high-resolution investigation of complex biological systems. These examples highlight how continuous workflow optimization and method development directly contribute to solving challenging structural and cellular research questions. 

What You Will Learn

• How integrated cryo-EM and correlative workflows enable the study of complex biological systems 

• Strategies for preserving delicate cell-cell interactions during high-pressure freezing 

• How cryo-fluorescence microscopy enables precise targeting of regions of interest for FIB milling

• How intracellular protein crystals can be accessed for electron crystallography without purification 

• How advanced instrumentation and facility expertise translate into high-impact scientific outcomes 

Who should attend 

• Life sciences researchers working with electron microscopy 
  

• Structural and cell biologists interested in cryo-EM and correlative approaches

• Core facility managers and advanced microscopy specialists

• Principal investigators planning technically challenging EM-based projects

Why You Should Attend

• Discover how advanced cryo-EM workflows support cutting-edge biological research 

• See real examples of technically demanding projects enabled by correlative and cryogenic EM

• Understand how workflow design and method development drive scientific innovation

• Gain inspiration for designing complex EM experiments in your own research environment

Overview of the Institute

The Institute of Molecular Genetics of the Czech Academy of Sciences (IMG Prague) is a leading life sciences research institute in Central Europe dedicated to understanding how cells, tissues, and organisms develop and function at the molecular level. IMG’s research spans molecular and cellular biology, genetics, immunology, virology, structural biology, bioinformatics, and related biomedical disciplines, with dozens of projects annually resulting in publications in prestigious international journals. 

As part of the Czech Academy of Sciences, IMG fosters close collaboration with Charles University in Prague and an extensive network of international partners. With state-of-the-art research infrastructure and expertise, it supports both fundamental discoveries and translational applications across diverse biological systems and experimental models. 

Core Facilities at IMG

IMG hosts a suite of state-of-the-art core facilities that provide open access to advanced technologies, expert support, and tailored services for both internal and external researchers. These facilities play a vital role in supporting multidisciplinary research by enabling high-resolution imaging, quantitative analysis, and integrated experimental workflows.

Key imaging technology platforms include:

• Electron Microscopy Core Facility - offering comprehensive ultrastructural imaging and sample preparation workflows, covering cryo-EM and volume EM methods, correlative microscopy, and elemental analysis. 

• Light Microscopy and Image Analysis - providing access to a broad spectrum of fluorescence and advanced light microscopy systems, image processing tools, and analytical expertise. 

• Flow Cytometry - delivering conventional and imaging cytometry, high-speed cell sorting, and analytical support.

These platforms strengthen research across cell biology, structural biology, immunology, virology, developmental biology, and other areas where high-performance imaging and data analysis are essential for generating new biological insights.

Register to join this research-focused session and gain practical insight into cryo-EM and correlative workflows for challenging biological projects.